Skip to main content
. Author manuscript; available in PMC: 2013 Dec 1.
Published in final edited form as: Virus Res. 2012 Oct 13;170(1-2):174–179. doi: 10.1016/j.virusres.2012.10.009

Figure 1. SAHA, VPA, TSA, NaB, and DZNep cause dose-dependent alterations in chromatin modification in feline cells.

Figure 1

SPF feline PBMC were treated with HDACi (A), HMTi (B), or no treatment (No Tx), and Western blots were performed on cell lysates (20 μg protein per lane) using a primary rabbit anti-acetylated histone (H3K9,14ac, Millipore) antibody (A) or anti-methylated histone (H3K27me3, Millipore) antibody (B) following normalization for total protein using a commercial BCA assay (Thermo Scientific). Band densities calculated using ImageJ (U.S. National Institutes of Health) were normalized to no treatment controls.