Skip to main content
. Author manuscript; available in PMC: 2013 Dec 1.
Published in final edited form as: Virus Res. 2012 Oct 13;170(1-2):174–179. doi: 10.1016/j.virusres.2012.10.009

Figure 4. SAHA-mediated FIV transcriptional activation is dose-dependent and not synergistic with DZNep.

Figure 4

CD4+ T-cells from 2 asymptomatically FIV-infected cats (cat 184 in A and B; cat 187 in C and D) were cultured ex vivo in the presence of varying concentrations of SAHA (100nM to 1μM), 5μM DZNep, a combination of 1μM SAHA and 5μM DZNep (SAHA + DZNep), no treatment (No tx), or mitogens. RNA was harvested from cells on days 0, 5, and 7 post-culture, and levels of FIV gag cDNA were normalized to cellular GAPDH cDNA (A and C). Values for day 5 are expanded in (B) and (D). Error bars represent the standard deviation of triplicate qPCR measurements, and one-way ANOVA followed by Dunnett’s multiple comparison post-test (GraphPad Prism) was used to determine if there was a significant difference relative to the no treatment control (ns - not significant, *p < 0.05, **p < 0.01, ***p < 0.001).