Figure 5. SAHA-mediated FIV activation results in virion formation and is not synergistic with DZNep.
CD4+ T-cells from asymptomatically FIV-infected cats 187 (A) and 184 (B) were cultured ex vivo in the presence of 1μM SAHA, 5μM DZNep, a combination of 1μM SAHA and 5μM DZNep (SAHA + DZNep), no treatment (No Tx), or mitogens. RT activity in clarified supernatants was calculated using a standard curve generated with a commercial RT assay kit (Roche). Error bars represent the standard deviation of triplicate RT assays, and one-way ANOVA followed by Dunnett’s multiple comparison post-test (GraphPad Prism) was used to determine significant differences relative to the no treatment control (ns - not significant, *p < 0.05, ***p < 0.001).