Skip to main content
. Author manuscript; available in PMC: 2014 Jan 1.
Published in final edited form as: J Steroid Biochem Mol Biol. 2012 Sep 12;133:1–11. doi: 10.1016/j.jsbmb.2012.09.004

Figure 5. 1,25-D3 down-regulates the expression of TIMP-3.

Figure 5

Cultures of C2C12 cells were treated as in Fig 3 for 24hs and 4 days. Total RNA and whole protein extracts were isolated for qRT-PCR and western blots respectively (A) Mean ± SEM corresponds to experiments done in triplicate, ***p<0.001 and western blots (B) and (C) Mean ± SEM corresponds to experiments done in triplicate, *p<0.05 and **p<0.01 at 24hs and 4 days respectively. Control-1, Control-2, VD1 and VD2 are different pools of two samples each. In both cases real time PCRs and western blots, samples and controls were normalized with GAPDH housekeeping gene.