TABLE 1.

Differential steady-state mRNA levels of angiogenic growth factors and angiogenic growth factor inhibitors between 1,25D3(100nM) treated and untreated C₂C₁₂ cells

			Fold Change
Gene Symbol	Description	Reference Sequence	24 hours	4 days	10 days
Fgf-1	Fibroblast growth factor 1	NM_008010	1.03	+5.77b	+11.47c
Vegfα	Vascular endothelial growth factor A	NM_009505	+3.28c	+6.55b	−1.09
Timp-3	Tissue inhibitor of metalloproteinase 3	NM_011595	−2.47c	−3.74b	−1.10
Fgf-2	Fibroblast growth factor 2	NM_008006	−2.13c	−2.06b	−1.87
Fgf-6	Fibroblast growth factor 6	NM_010204	+1.13	+1.35	+1.13

Total RNA from cells treated with or without 1,25-D3 for 24 hours, 4 days and 10 days was subjected to RT real time PCR by the Angiogenic Growth Factor and Angiogenesis Growth Factor Inhibitor arrays, and the ratios between the treated 1,25D3-treated and 1,25D3-untreated cells were corrected by GAPDH, Hprt1, Hsp90ab1, and Actb were calculated for assays performed in triplicate. Experiments were performed in triplicate.

^aP < 0.05

^bP < 0.01

^cP < 0.001