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. Author manuscript; available in PMC: 2012 Dec 4.
Published in final edited form as: Stem Cells. 2008 May 22;26(8):2042–2051. doi: 10.1634/stemcells.2008-0149

Figure 1. Non-Adherent Bone Marrow Fractions Alter CFU-F/CFU-OB Formation.

Figure 1

Non-adherent bone marrow cell fractions (NABM) were evaluated for their effects on the generation of fibroblastic (CFU-F) and osteoblastic (CFU-OB) colonies from BMSCs in vitro. Cells from primary bone marrow stromal cell (BMSC) cultures were trypsinized, resuspended and replated at 100 cells/well. NABM cells were collected from marrow and plated on plastic for 24 hours, recovered and washed, and placed into the top chamber of a chambered culture plate to exclude the possibility that cells from the non-adherent bone marrow fraction may contribute to colony formation. Newborn dermal fibroblasts (DF) were placed into the top chamber of the culture to served as negative controls. Cultures of just the NABM were also included. After 21 days the colonies (>50 cells) were quantified as either CFU-F or CFU-OB using methyl violet or Von Kossa staining. The data are presented as mean ± s.d. for n=6 determinations in three independent investigations. *p <0.05 compared to no cells in the top well (Students t-test).

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