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HSCs (Sca-1+cKit+CD150+CD41−CD48−) were isolated 2 days following an acute stress from young (4 weeks), sexually mature (12 weeks) and aged (5–7 months) mice. The cells were lysed and RNA prepared for evaluation by real-time RT- PCR. The data are presented as fold change normalized to GAPDH, where the expression levels of the HSCs derived from the non-stressed animals was set as the standard. *p < 0.05 versus the non-stressed animals.
