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. Author manuscript; available in PMC: 2012 Dec 4.
Published in final edited form as: Cell Rep. 2012 Nov 8;2(5):1096–1103. doi: 10.1016/j.celrep.2012.10.007

Figure 3. STN1 promotes C-strand fill-in.

Figure 3

(A) Strategy to monitor C-strand fill-in at lagging daughter telomeres. (B-D) Analysis of overhang density. HeLa 1.2.11 clones were labeled with BrdU for the indicated times after release into S. Overhangs were released from lagging daughters with DSN and analyzed in CsCl gradients. (B) Detection of overhangs in gradient fractions by slot blot with probe to the overhang. (C) Quantification of overhang signals from shNT or shSTN1-7 clones plotted versus density (representative experiment). Data are from blots shown in (B). (D) As for (C) but with shSTN1 cells rescued with sh-resistant STN1.