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. Author manuscript; available in PMC: 2013 Dec 1.
Published in final edited form as: Best Pract Res Clin Haematol. 2012 Oct 23;25(4):409–414. doi: 10.1016/j.beha.2012.10.002

Figure 2. Sequencing of HSPC clones from healthy donors.

Figure 2

A. Peripheral blood or umbilical cord mononuclear cells (MNCs) from 7 healthy donors were isolated, and an aliquot was reserved for exome sequencing. CD34+ CD38- lineage- hematopoietic stem/progenitors cells (HSPCs) were sorted into a 96-well plate and expanded for 3 weeks on stromal cells with a cocktail of cytokines. Three individual clones were selected for exome sequencing for each donor. B. Shown is the number of single nucleotide mutations per exome for the 7 healthy donors. The 2 umbilical cord donors are designated with an age of zero.