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. Author manuscript; available in PMC: 2012 Dec 4.
Published in final edited form as: Biochim Biophys Acta. 2010 Jul 21;1804(11):2136–2145. doi: 10.1016/j.bbapap.2010.07.013

Fig. 1.

Fig. 1

Monitoring urea-mediated denaturation of Magnaporthe grisea KatG1 (A, C, E) and Synechocystis KatG (B, D, F) by heme Soret spectroscopy. (A) Spectral transition of 2.2 μM recombinant Magnaporthe grisea KatG1 in 5 mM phosphate buffer, pH 7.0, mediated by various urea concentrations (incubation time 18 h at 25 °C): 0 M (gray line), 1.5, 2, 2.5 and 5 M). (B) Spectral transition of 3.8 μM recombinant Synechocystis KatG in 5 mM phosphate buffer, pH 7.0, mediated by various urea concentrations (incubation time 18 h at 25 °C): 0 M (gray line), 1.0, 1.5, 2.0 and 3.5 M urea). (C, D) Plots of Soret maximum and absorbance at 408 nm in dependence of urea concentration. Conditions as in (A, B). (E, F) Standard free enthalpies as a function of urea concentration. ΔG° values have been calculated from plots of absorbance at 408 nm versus urea concentration as described in Materials and methods.