Fig. 4.
Thermal stability of the heme cavity of Magnaporthe grisea KatG1 and Synechocystis KatG as monitored by electronic absorbance spectroscopy. Change in heme Soret spectra during thermal denaturation of MagKatG (A) and SynKatG (B). (C, D) Change in Soret maximum (●) and decrease of Soret intensity (○, at 408 nm) of Magnaporthe grisea KatG (8.7 μM) and Synechocystis KatG (7.95 μM) in 5 mM phosphate buffer, pH 7.0, containing 0.5 M urea. (E, F) Van’t Hoff plots and fits.
