Fig. 6.
Differential scanning calorimetry of Magnaporthe grisea KatG1 and Synechocystis KatG and dimeric structure of KatG. Normalised DSC thermograms of MagKatG1 (A) and SynKatG (B) after pre and post-transitional baseline subtraction. Conditions: 4 μM enzyme in 5 mM phosphate buffer, pH 7.0, containing 0.5 M urea. Fit of experimental data points (gray line) to a non-two-state model with two unfolding transitions shown in black. (C) Dimeric structure of Burkholderia pseudomallei KatG (PDB code: 1MWV). Blue and orange: heme-containing N-terminal domains, red and green: C-terminal domains. Figure was constructed with PyMOL.
