Table 4.

Summary of USC subcutaneous injection in vivo for 28 days

Groups	1	2	3	4	5	6	7	8	9	10
	Cell-free + empty beads	Human Myoblasts +empty beads	USC+ empty beads	USC/EC+ empty beads	Cell free +all growth factor beads	USC+ PDGF/HGF beads	USC+ VEGF Beads	USC+IGF/ FGF/NGF Beads	USC+All growth factor containing beads	USC/EC+All growth factor containing beads
Gross measurement
Graft size	2+	3+˜4+	3+	3+	3+	4+	3+˜4+	4+	3+˜4+	4+
Blood supply	−	+˜2+	2+	2+	2+˜3+	3+	3+˜4+	2+˜3+	4+	4+
Cell density
DAPI staining	+	3+˜4+	2+˜3+	2+˜3+	2+	3+	4+	3+˜4+	4+	4+
Human nuclei staining	−	3+	2+	2+˜3+	−	3+	3+˜4+	3+	3+˜4+	4+
Muscle regeneration
MyoD	−˜+	3+˜4+	2+	2+	2+	3+˜4+	3+	2+˜3+	3+˜4+	4+
Desmin	−	4+	2+	+˜2+	2+	4+	2+˜3+	3+	4+	4+
Myf-5	−	4+	+˜2+	2+	2+˜3+	3+	2+˜3+	2+	3+˜4+	4+
Angiogenesis
Trichorome observation	+	2+˜3+	2+	2+	+˜2+	3+	3+˜4+	3+	4+	4+
CD 31	−˜+	2+	2+˜3+	2+	2+˜3+	3+	3+˜4+	3+	3+˜4+	4+
vWF	−˜+	+˜2+	2+	2+˜3+	2+˜3+	2+˜3+	3+	3+	4+	4+
Nerve regeneration
S-100	−˜+	+˜2+	+˜2+	+	2+	+˜2+	2+	3+	4+	4+
NF	−˜+	2+	2+	+˜2+	2+	2+	2+˜3+	3+˜4+	3+˜4+	4+

Notes: All growth factors including PDGF-BB/HGF/VEGF/IGF/FGF-1/NGF.

− no targeted cell or capillary; + 1%–25% of G10 changes; ++ 25%–50% of G10 changes; +++ 50%–75% of G10 changes; ++++ G10 changes as standard