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. 2011 Aug;90(2):357–366. doi: 10.1189/jlb.1210702

Figure 1. CMC and HIES patients have defective IL-17 production upon C. albicans stimulation.

Figure 1.

(A) PBMCs from CMC patients (n=3), consisting of a coculture of monocytes and lymphocytes, and healthy controls were stimulated with heat-killed C. albicans for 24 h. Supernatant was harvested, and TNF and IL-6 concentration was determined by ELISA (n≥6, *P<0.05). (B) PBMCs from CMC (n=3) and HIES (n=4) patients and healthy control were stimulated with heat-killed C. albicans for 7 days. Supernatant was harvested, and IL-17 and IL-22 concentration was determined by ELISA. (C) Intracellular cytokine staining of IL-17 and IFN-γ in human PBMCs stimulated for 5 days with RPMI or C. albicans and then stimulated for 4 h with PMA and ionomycin. Cells were gated for CD4. Representative data from two separate experiments with a total n = 3 are shown here.