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. Author manuscript; available in PMC: 2012 Dec 4.
Published in final edited form as: J Immunol. 2009 Mar 15;182(6):3414–3422. doi: 10.4049/jimmunol.0801430

FIGURE 2.

FIGURE 2

Normal lymphocyte composition in the BM of lpr and gld mice. The same animals described in Figure 1 were also analyzed for total BM cells per mouse (Vicell counter, Beckman Coulter, FL) and proportions of BM CD4+ and CD8+ T cells by FACS analysis (A). BM cells were stained with the antibody mixture Lin (CD3, CD4, CD8, CD11b CD45R, Gr1, Ter119)-PE + CD117-APC, + CD34-FITC for the proportion of Lin−Sca1+Kit+ (LSK) cells (B), and with CD25-PE + FoxP3-PE-Cy5 + CD4-APC for the proportion of Treg cells (C).