FIG. 2.

Percentage of MT-G-positive sperm cells. After the appropriate incubations, spermatozoa were loaded with MT-G (20 μM) in Tyrode's medium, incubated for 20 min, and evaluated by a dual-channel confocal microscope. MT-G-positive (arrow) and MT-G-negative (dotted arrow) spermatozoa were observed. The values represent the mean±standard error of the mean (SEM) for five samples per group. For each sample, 200 cells were evaluated in three different fields, and representative staining was shown. Compared with the control, ***p<0.001; compared with the untreated group, ^###p<0.001. Magnification: ×63, orig. (To see this illustration in color the reader is referred to the web version of this article at www.liebertpub.com/ars.) MT-G, MitoTracker Green FM.