FIG. 4.

Detection of ^•NO. Concentration of ^•NO in sperm cells incubated in Tyrode's medium supplemented or not with 50 μM M40403 SOD mimic, evaluated immediately or after 3 h of incubation. ^•NO content was assessed electrochemically, by the inNO-II ^•NO measurement system, using the amino-700 sensor electrode. Relative ^•NO concentrations are presented as percentages of the control, which was taken as 100%. Data represent the mean±SEM (n=30, 10 per group). Compared with the control, **p<0.01; compared with the untreated group, ^###p<0.001. SOD, superoxide dismutase; ^•NO, nitric oxide.