FIG. 8.

Purity of mRNA samples. Agarose gel showing the PCR product for specific markers of leukocytes (CD45), germ cells (c-kit), and epithelial cells (E-cad) used as positive controls in lines 4, 8, and 12, respectively. Each sperm sample from all examined groups was examined for the presence of CD45, c-kit, and E-cad. PCR products of all samples per each group are coupled and loaded onto lines: 1, 5, 9—control group; 2, 6, 10—untreated group; 3, 7, 11—SOD mimic-treated group; Each of the examined samples showed the absence of RNA from contaminating cells (lines 1–4, CD45; 5–8, c-kit; 9–12, E-cad). Monocytes and endometrial epithelial cells were used as positive markers of CD45 and E-cad, respectively. Human semen samples contaminated by testicular germ cells were used as positive controls for the c-kit.