FIG. 8.

Effect of RSV infection on ARE-driven gene transcription. A549 cells were transiently transfected with a plasmid containing multiple copies of the NQO1 ARE site linked to the luciferase gene and then infected with RSV. Cells were harvested at different times p.i. to measure luciferase activity. Uninfected plates served as controls. For each plate, luciferase was normalized to the β-galactosidase reporter activity. Data are expressed as mean±standard deviation of normalized luciferase activity. ARE, antioxidant-responsive element; NQO1, quinone oxidoreductase.