Fig. 6.

Heterogeneity of colon tumour cells in Apc^Min/+ mouse. (A) Double immunostaining for β-catenin (red) and GFP (green) in the colon tumour of Apc^Min/+ mouse with transgenic GFP reporter allele for β-catenin/Tcf transcription activity. Note that heterogeneous expressions of both β-catenin and GFP are observed in a colon tumour. (B) Double immunostaining for β-catenin and Ki-67 in a colon tumour. Tumor cells with strong β-catenin expression show less frequent staining for Ki-67. (C) Double immunostaining for Hes1 (green) and Ki-67 (red). Distinct localisation of Hes1-expressing cells and Ki-67-positive cells are seen in colon tumour. Arrows indicate cells with positive nuclear staining for Hes1. (D) Immunostaining for β-catenin and Hes1 in serial sections. Colocalisation of higher levels of β-catenin and Hes1 expression is observed in the colon tumour.