Fig. 4.

Reduced Notch signaling restores photoreceptor cell fate in Abl clones. (A-D) Adult eyes carrying Abl clones in wild-type (A), N^264-39/+ heterozygous (B) or Su(H)²/+ heterozygous (C) genetic backgrounds were stained with anti-Elav (red) and anti-GFP (green). All images are maximal projections of optical sections spanning the whole disk. (A) Few Elav⁺ cells remain in Abl² clones. (B,C) More Elav⁺ cells are evident in N^264-39/+; Abl² (B) and Su(H)²/+; Abl² (C) clones. (D) Quantification of Elav⁺ cell density in N^264-39/+; Abl² and Su(H)²/+; Abl² clones. Seven to eight clones per genotype were scored, with values plotted as mean ± s.d. (E-G) Dissected eyes from newly eclosed GMR-Gal4; tub-Gal80^ts >UAS-NICD adults stained for Elav and DAPI. (E) The 18°C raised control has an organized Elav⁺ nuclear lattice. (F) Eyes from animals shifted to 25°C at the white prepupal stage have fewer Elav⁺ cells but normal DAPI-stained cell density. (G) Quantification of Elav⁺/DAPI⁺ ratios. Three to five clones per genotype were scored, with values plotted as mean ± s.d. Scale bars: 10 μm.