Figure 1.

Azide restores OE17 processing in the presence of uncouplers. Where indicated, isolated intact chloroplasts were preincubated with 30 mm azide, 15 μm CCCP, and 6 μm nigericin (or equivalent volumes of water or ethanol) for 10 min in the dark on ice. To start the reactions, the chloroplasts were added to the import reaction mixture (final concentrations of inhibitors were 10 mm azide, 5 μm CCCP, and 2 μm nigericin) and placed in light for 30 min. Reactions were terminated by centrifuging into PCA as described in Methods. The standard (std) represents 20% of the prOE17 added to each reaction; the positions of the precursor (p), intermediate (i), and mature (m) proteins are noted.