Figure 5.

Intact chloroplasts were preincubated with OAA/DHAP (3 mm), azide (30 mm), nigericin (6 μm), or equivalent volumes of 1× IB, water, or ethanol, respectively, for 10 min on ice in the dark. Reactions were initiated by the 3-fold dilution of chloroplasts into the import mixture and continued for 30 min in the light. The assay was terminated and samples were analyzed as described in Figure 1. The standard (std) represents 20% of the precursor added to each reaction; the positions of the precursor (p), intermediate (i), and mature (m) proteins are noted.