Table 2.
List of Primers and Their Sequences Used for Real-Time Polymerase Chain Reaction Studies
| Gene | Species | Accession | Left primer | Right primer |
|---|---|---|---|---|
| Nrf2 | Mouse | NM_010902.3 | CATGATGGACTTGGAGTTGC | CCTCCAAAGGATGTCAATCAA |
| Nrf2 | Rat | NM_031789.1 | AGCATGATGGACTTGGAATTG | CCTCCAAAGGATGTCAATCAA |
| Gclm | Mouse | NM_008129.3 | TGACTCACAATGACCCGAAA | GATGCTTTCTTGAAGAGCTTCCT |
| Gclm | Rat | NM_017305.2 | CTGACTCACAATGACCCAAAAG | TTCAATGTCAGGGATGCTTTC |
| Gclc | Mouse | NM_010295.1 | ATGATAGAACACGGGAGGAGAG | TGATCCTAAAGCGATTGTTCTTC |
| Gclc | Rat | NM_012815.2 | GGCGATGTTCTTGAAACTCTG | CAGAGGGTTGGGTGGTTG |
| Nqo-1 | Mouse | NM_008706.5 | AGCGTTCGGTATTACGATCC | AGTACAATCAGGGCTCTTCTCG |
| Nqo-1 | Rat | NM_017000.3 | AGCGCTTGACACTACGATCC | CAATCAGGGCTCTTCTCACC |
| Ho-1 | Mouse | NM_002133.2 | GGGTGATAGAAGAGGCCAAGA | AGCTCCTGCAACTCCTCAAA |
| Ho-1 | Rat | NM_012580.2 | GTCAAGCACAGGGTGACAGA | CTGCAGCTCCTCAAACAGC |
| Gsr | Mouse | NM_010344.4 | AGTTCCTCACGAGAGCCAGA | CAGCTGAAAGAAGCCATCACT |
| Gapdh | Mouse | NM_008084.2 | GCCAAAAGGGTCATCATCTC | CACACCCATCACAAACATGG |
| Gapdh | Rat | NM_017008.3 | TGGGAAGCTGGTCATCAAC | GCATCACCCCATTTGATGTT |
| Tnf | Mouse | NM_013693.2 | TCTTCTCATTCCTGCTTGTGG | GGTCTGGGCCATAGAACTGA |
| Cd68 | Mouse | NM_009853.1 | GACCTACATCAGAGCCCGAGT | CGCCATGAATGTCCACTG |
| Ccl2 | Mouse | NM_011333.3 | CATCCACGTGTTGGCTCA | GATCATCTTGCTGGTGAATGAGT |
Cd68, cluster of differentiation 68; Gapdh, glyceraldehyde-3-phosphate dehydrogenase; Gclc, glutathione cysteine ligase regulatory subunit; Gclm, glutathione cysteine ligase modulatory subunit; Gsr, glutathione reductase; Ho-1, hemeoxygenase 1; Ccl2, (Mcp1) monocyte chemotactic protein-1; Nqo1, NADPH quinone oxidoreductase 1; Nrf2, nuclear factor E2-related factor 2; Tnf, tumor necrosis factor.