Figure 7.

Nucleotide exchange activity of Sse1 is required for proper spindle assembly. (A) 10× serial dilutions of log-phase sse1Δmad2Δ cells harboring either empty vector, pSSE1, pSSE1-K69Q, or pSSE1-G233D plasmid were spotted onto YPD and incubated at 30°C for 2 d. (B) Logarithmically growing sse1Δ cells expressing Spc42-RFP as an SPB marker and harboring either empty vector, pSSE1, pSSE1-K69Q, or pSSE1-G233D plasmid were arrested in S phase with 100 mM HU for 2.5 h at 30°C. Spindle length was measured using Spc42-RFP fluorescence. The data shown are from a single representative experiment out of three repeats. (C) 10× serial dilutions of log-phase sse1Δmad2Δ cells harboring either empty vector, pSSE1, pFES1, or pSNL1ΔN plasmid were spotted onto YPD and incubated at 30°C for 2 d.