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. 2012 Aug 20;198(4):561–574. doi: 10.1083/jcb.201111136

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© 2012 Thon et al.

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Figure 4. — TLR9 colocalizes with PDI to electron-dense membrane-encapsulated regions adjacent the plasma membrane along the periphery of human PLTs. (A) Representative maximum projection z series for TLR9 and PDI by confocal immunofluorescence microscopy demonstrating colocalization. (B–E) Washed human whole-blood PLTs were fixed, frozen, and sectioned before mounting on Formvar carbon-coated copper grids. Ultrathin PLT sections were probed for TLR9, and bound antibody was labeled with immunogold. Samples were examined by electron microscopy and reveal the distribution of TLR9 (B), PDI (C), and colocalization of both (D and E) along the periphery of resting human PLTs. White arrows denote localization of TLR9 and PDI to limiting membrane of electron-dense regions adjacent the plasma membrane. (D and E) For colabeling, immunogold particles are 15 nm for PDI and 10 nm for TLR9.