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. 2012 Aug 20;198(4):529–544. doi: 10.1083/jcb.201201153

Figure 2.

Figure 2.

The middle Q-rich domain is not required for GW/P-body formation. The N-terminal polyQ domain (1–253 aa), the middle Q-rich domain (1,151–1,490 aa), and the C-terminal silencing domain (1,491–1,962 aa) cannot nucleate the de novo formation of GW/P bodies. Deletion of the middle Q-rich domain does not affect the ability of GW220 (GWΔQ rich) to nucleate the formation of GW/P bodies. The presence of myc-tagged GW proteins and endogenous components of GW/P body were determined using the indicated antibodies. The expression levels of the myc-tagged truncated domains of the GW220 proteins (indicated by asterisks) were determined by Western blot analysis using the myc antibody. The expression levels of full-length GW220 and GW(ΔQ rich; indicated by asterisks) were also determined by Western blot analysis using an antibody that recognizes the C terminus of the TNRC6A/GW proteins. RRM, RNA recognition motif. Bars, 10 µm.