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. 2012 Aug 20;198(4):529–544. doi: 10.1083/jcb.201201153

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© 2012 Castilla-Llorente et al.

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Figure 4. — mRNA targeted by miRNA accumulates in endogenous GW/P bodies in the U2OS cells and is retained in the endogenous GW/P bodies after either emetine or cycloheximide treatment. A β-globin reporter construct with a 3′ UTR containing both the miRNA target sites and 24 binding sites for the MS2 coat protein allowed the tracking of the reporter mRNA by coexpression of the MS2-YFP-NLS fusion protein. In the absence of miRNA, the MS2-YFP protein mostly localized to the nucleus as a result of the presence of an NLS. In the presence of an miRNA, MS2-YFP protein became concentrated into the foci marked by endogenous GW220. Bars, 10 µm.