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. 2012 Aug 20;198(4):529–544. doi: 10.1083/jcb.201201153

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© 2012 Castilla-Llorente et al.

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Figure 5. — GW/P bodies are stable mRNP granules in vitro and are insensitive to RNase treatment. (A) Stable GW/P bodies were present in the in vitro RISC extract, determined by immunostaining using antibodies against Hedls, GW220, Ago2, and DDX6. (B) GW/P bodies extracted from U2OS cells were insensitive to in vitro RNase treatment, determined by immunostaining using antibodies against Hedls, Ago2, and GW220. (C) Classical P bodies extracted from HeLaS cells were unstable and disappeared completely after in vitro RNase treatment, determined by immunostaining using antibodies against DDX6 and Hedls. Bars, 10 µm.