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. 2012 Aug 20;198(4):677–693. doi: 10.1083/jcb.201202094

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© 2012 Elbediwy et al.

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Figure 2. — SH3BP1 regulates epithelial junction formation. (A–F) Caco-2 cells transfected with siRNAs were plated in low calcium medium before adding calcium to induce junction formation, which was followed by staining with fluorescent phalloidin (A), ZO-1 (B), E-cadherin (C), p120 catenin (D), or by measuring transepithelial electrical resistance (TER; E). (F) After 48 h, paracellular tracer diffusion was measured. E and F show means ± 1 SD; n = 3. (G and H) siRNA-transfected A431 cells were plated in low calcium medium and then stimulated for different periods of time with calcium to induce junction formation. Shown are images of cells stained phalloidin (G) or with anti–E-cadherin antibodies (H). Note that depletion of SH3BP1 leads to a strong induction of E-cadherin–positive filopodia. Bars, 10 µm. p, phosphorylated.