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. 2012 Aug 20;198(4):711–730. doi: 10.1083/jcb.201203099

Figure 1.

Figure 1.

Stt4 and Mss4 are required for filamentous growth. (A) Mss4 and Stt4 are necessary for hyphal growth. Indicated strains were incubated in the presence (120 min) and absence of FCS with and without Dox (ni.ex. = 5). (B) Quantification of hyphal growth defects. Cells were incubated with FCS as above and the percentage of filamentous cells determined (5 times 50 cells). (C) The stt4 mutant elongates, yet is unable to form hyphae. Cells were incubated with FCS as above and lengths determined (ni.ex. = 2; 125 cells each). (D) An mss4 mutant specifically defective in invasive filamentous growth. Indicated strains were incubated on FCS containing agar at 30°C for 8 d (ni.ex. = 3). (E) Quantitation of colony filament length in mss4-f12 mutant cells. Filament length was determined (ni.ex. = 3) and averages shown (wt: 100% = 1.1 mm).