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. 2012 Aug 20;198(4):711–730. doi: 10.1083/jcb.201203099

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© 2012 Vernay et al.

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Figure 5. — Sufficient PI(4)P is necessary for PI(4,5)P₂ asymmetry. (A) Asymmetric PI(4,5)P₂ distribution requires normal PI(4)P levels. Images of representative cells, as described in Fig. 4 A. Stt4 cells expressing the PI(4,5)P₂ reporter were grown in the presence or absence of Dox (n_i.ex. = 3). (B) Quantification of PI(4,5)P₂ concentration over long axis of budding cells. Average signal concentration over the long axis of small budded cells from A is shown with SD in gray, as described in Fig. 4 A (n = 15 cells, individual profiles shown in Fig. S3 A). (C) Reduction of Stt4 levels does not affect PI(4,5)P₂ reporter levels. Immunoblot showing the levels of the reporter (α-GFP) and loading control (α-Cdc11) in the strains used in A.