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. 2012 Aug 20;198(4):711–730. doi: 10.1083/jcb.201203099

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© 2012 Vernay et al.

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Figure 8. — Mss4 localizes to small buds and tips of hyphal filaments. (A) Mss4 is localized in a tight cluster in small buds. A mss4 strain expressing GFP-Mss4 was grown in the presence of Dox. Central z-sections and sum projections (12 z-sections) of representative cells are shown (top) and quantification of Mss4 concentration over long axis of cells (bottom) as described in Fig. 4 A (n_i.ex. = 3). (B) Mss4 is localized in a tight cluster at hyphal filament tips. Cells were incubated in the presence of FCS as indicated. Images of representative cells as described in A. (C) Quantification of Mss4 concentration over long axis of filamentous cells. Average signal concentration over the long axis of cells incubated with FCS as described in Fig. 4 A (n = 43 cells from B) shown with SD in gray (left) and comparison of PI(4,5)P₂ and Mss4 distribution (right) as in Fig. 4 A (n = 43 cells from B and 25 cells from Fig. 4 C). Maximum signals for PI(4,5)P₂ and Mss4 were normalized to 100%. (D) PI(4,5)P₂ and Mss4 localization in filamentous cells. wt cells expressing yemCh PI(4,5)P₂ reporter or GFP-Mss4 were mixed and then treated with FCS for 30 min. Sum projections (8 z-sections) of representative cells as in Fig. 4 B.