Table I.
Experiment | UMPSase
Activitya
|
Hybridization | Radioactivity
Bound
|
||||||
---|---|---|---|---|---|---|---|---|---|
Uninduced | FOA-Induced | Uninduced | FOA-Induced | ||||||
milliunit | % | milliunit | %b | cpm | % | cmp | %c | ||
1 | 330 | 100 | 714 | 216 | 1 | 43 | 100 | 101 | 234 |
2 | 94 | 100 | 206 | 220 | |||||
2 | 208 | 100 | 625 | 251 | 3 | 371 | 100 | 960 | 259 |
4 | 736 | 100 | 928 | 126 | |||||
5 | 1148 | 100 | 2658 | 232 | |||||
6 | 573 | 100 | 2035 | 355 | |||||
3 | 270 | 100 | 672 | 248 | 7 | 47 | 100 | 93 | 198 |
8 | 90 | 100 | 196 | 218 | |||||
9 | 85 | 100 | 246 | 290 |
Activity is expressed in milliunits per milligram of protein. One unit is defined as the amount of enzyme activity that liberates 1 nmol of CO2 per min at 25°C.
Average (±sd) was 238 ± 19 (n =3).
Average (±sd) was 237 ± 59 (n = 9).