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Effect of pyrimidines on 5-fluorouracil induction of UMPSase
| Treatment | UMPSase Activity | Control |
|---|---|---|
| milliunits/mg protein | % | |
| None | 234 ± 7 | 100 |
| 5-FU | 497 ± 9 | 212 |
| 5-FU + thymidine | 144 ± 1 | 62 |
| 5-FU + cytidine | 339 ± 10 | 145 |
Cells were grown on Murashige-Skoog solid media supplemented with 400 μm 5-fluorouracil (5-FU) alone or 400 μm 5-fluorouracil plus 400 μm nucleotide for 12 d. Then all cells were frozen at −70°, thawed, and assayed for UMPSase activity as described in Methods (n = 4).
