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Colony-forming efficiency of organic solvent-tolerant mutants from E. coli JA300 on LBGMg agar medium in the absence of an organic solvent (A) and in the presence of cyclohexane (B) or cyclohexane and p-xylene (7:3 vol/vol mixture) (C). Each strain was spotted at a tenfold dilution and incubated at 25°C for 48 h.
