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. 2012 Nov 14;5:261. doi: 10.1186/1756-3305-5-261

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Copyright ©2012 Nico et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Assessment of the IFN-γ cytokine secretion by splenocytes. IFN-γ was assayed in the supernatants of splenocytes of C57 and KOB2 infected mice and in uninfected C57 and KOB2 control mice after 5 days of in vitro culture with the freeze and thawed lysate of Leishmania (L.) chagasi promastigotes. Data correspond to individual results of one experiment with 6 mice of each group obtained after sacrifice. In graph A, horizontal full lines represent the mean values of C57 normal uninfected (C57), C57 infected (C57i), KOB2 normal uninfected (KOB2) and KOB2 infected (KOB2i) mice. *p<0.05 indicates significant differences to the saline control as disclosed by the Kruskall Wallis (KW) and Mann Whitney (MW) non-parametrical tests. The results were expressed on graphs as scattering of individual values. R squared (R²) (coefficients of determination) estimates are shown on graphs. Trend lines were added.