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. 2012 Dec 4;7(12):e51109. doi: 10.1371/journal.pone.0051109

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© 2012 Pelosi et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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A- Immunophenotypic characterization of adherent cells generated by CD34+ cells cultured in MH-CM for 30 days. Mean values +/− SD of 15 independent experiments are shown. B- Flow cytometry analysis of the indicated endothelial markers expressed by adherent cells generated by CD34+ cells cultured in MH-CM for 30 days. Cells have been labelled with CD45, CD144 and with the indicated antigen. Shown are plots of CD45 negative cells (87+/−9%). The percentage of double-positive cells for the two endothelial antigens are given. A representative experiment out of 15 is shown. C- Confocal imaging: triple immunostaining analysis for the indicated antigens of adherent cells generated by CD34+ cultured in MH-CM for 30 days. A representative experiment out out of 5 is shown.