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. 2012 Dec 4;7(12):e51109. doi: 10.1371/journal.pone.0051109

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© 2012 Pelosi et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Schematic representation of the various stages of haemogenic endothelium differentiation towards haemopoietic cells. B- Top Clonogenetic assay of sorted CD144+45− cell populations in unicellular liquid culture in the presence of endothelial, MH-CM and haematopoietic medium. -Middle Clonogenetic assay of sorted CD144+45+ cell populations in unicellular liquid culture in the presence of MH-CM and haematopoietic medium. Single cells (at a 0,25 cell/well dilution) were seeded in the different media: the number and the morphology of colonies scored after 20 days. Three independent experiments are reported. - Bottom: Confocal double immunofluorescence analysis for CD45 (green) and CD144 (red) antigen expression in endothelial, haemat.-endot. and haematopoietic colonies grown in unicellular liquid culture in the presence of the different media described above.