Figure 2.

Addressable peptide arrays reveal SH2 domain selectivity. (A) 50 SPOT arrays panned against 50 GST-SH2 domains reveals the highly selective nature of SH2 domain phosphopeptide interactions. Interactions were detected using anti-GST antisera and Alexa Fluor-680-labeled anti-goat secondary antibody and the intensity of signals recorded using LiCor Odyssey. (B) Two separate peptide arrays were probed with independent SH2 domain preparations for three SH2 domains (SHB, SHIP2, SH3BP2). The scatter plot reveal some variability between the independent SPOT experiments yet revealing a strong correlation coefficient (R²).