Figure 10. tTAV2 expression in homozygous OX3402C pink bollworm.

Amount of tTAV2 mRNA in extracts from larvae and adults, determined by real-time PCR. Data are normalised to the level of tTAV2 RNA in OX3402C-homozygous larvae reared on CTC. Analysed samples were second-instar larvae fed CTC or non-CTC diet, and the following homozygous OX3402C adults (reared as larvae on diet with CTC): newly eclosed adults (<12 h after eclosion; ‘NE adult’); 2-day-old adults with CTC or non-CTC sugar water; 4-day-old adults with CTC or non-CTC sugar water; and 6-day-old adults with CTC or non-CTC sugar water. All samples underwent three technical replicates. For adults, biological replicates consisted of three single moths per sample category. For larvae on and off CTC, which each comprised one pooled sample of >10 larvae, technical replicates were used to calculate mean relative quantity. In adult samples, mean relative quantity between technical replicates was used to calculate mean relative quantity between biological replicates. No RT, no reverse transcriptase control reaction; dH₂O, water control. Error bars represent standard error of the mean (between technical replicates for larvae on CTC; between biological replicates for adult samples).