Table 1.
Genes |
Accession number |
Primer sequences |
Amplicon size (bp) |
||
---|---|---|---|---|---|
Forward (5’ → 3’) | Reverse (5’ → 3’) | ||||
Cell Surface Markers |
|
|
|
||
CD29 |
AF349461 |
GTGCCCGAGCCTTCAATAAAG |
CCCGATTTTCAACCTTGGTAATG |
87 |
|
CD34 |
AB021662 |
TGGGCATCGAGGACATCTCT |
GATCAAGATGGCCAGCAGGAT |
107 |
|
CD45 |
NM_001206523 |
CCTGGACACCACCTCAAAGCT |
TCCGTCCTGGGTTTTATCCTG |
101 |
|
CD73 |
BC114093 |
TGGTCCAGGCCTATGCTTTTG |
GGGATGCTGCTGTTGAGAAGAA |
115 |
|
CD90 |
BC104530 |
CAGAATACAGCTCCCGAACCAA |
CACGTGTAGATCCCCTCATCCTT |
96 |
|
CD105 |
NM_001076397 |
CGGACAGTGACCGTGAAGTTG |
TGTTGTGGTTGGCCTCGATTA |
115 |
|
Differentiation Markers |
|
|
|
||
PPARG |
NM_001100921 |
GCCCTGGCAAAGCATTTGTA |
TGTCTGTCGTCTTTCCCGTCA |
94 |
|
SCD1 |
AJ001048 |
CCCAGCTGTCAGAGAAAAGG |
GATGAAGCACAACAGCAGGA |
115 |
|
IL6 |
FJ409227.1 |
CAGCAAGGAGACACTGGCAG |
TGATCAAGCAAATCGCCTGAT |
101 |
|
COL1A1 |
AF129287 |
CCTGCGTACAGAACGGCCT |
ACAGCACGTTGCCGTTGTC |
93 |
|
BGLAP |
DQ418490 |
CCCAGGAGGGAGGTGTGTG |
CTAGACCGGGCCGTAGAAGC |
99 |
|
BGN |
NM_001009201.1 |
AACATGAACTGCATTGAGATGGG |
GCGAAGGTAGTTGAGCTTCAGG |
93 |
|
LUM |
NM_173934.1 |
AAGCAATTGAAGAAGCTGCACA |
TTAGTGAGCTGCAGGTCCACC |
92 |
|
NES |
194665083 |
CAAATCGCCCAGGTCCTG |
GCCTCTAGGAGGGTCCTGTATGT |
95 |
|
NEFM |
194669578 |
GCTCGTCATCTGCGAGAATACC |
CACCCTCCAGGAGTTTCCTGTA |
91 |
|
NELF |
27806522 |
CGCTATGCAGGACACAATCAAC |
GGGTCTCCTCACCTTCCAAGA |
161 |
|
TUBB3 |
116004470 |
GACCTCGAGCCTGGAACCAT |
GCCCCACTCTGACCAAAGATG |
92 |
|
MAP2 |
194664873 |
TGTCCCAGTGGAGGAAGGTTT |
TCTTGTCTAGTGGCTCGGCTG |
95 |
|
PRNP |
BC119821 |
CGCAGAAGCAGGACTTCTGAA |
TGGATTTGTGTCTCTGGGAAGA |
86 |
|
Housekeeping genes |
|||||
G6PDH2 |
AJ507200 |
TGACCTATGGCAACCGATACAA |
CCGCAAAAGACATCCAGGAT |
76 |
|
HPRT3 | EF078978 | AGGTGTTTATTCCTCATGGAGTAATTATG | GGCCTCCCATCTCCTTCATC | 79 |
GenBank accession numbers of the sequences used for primer design. Primer sequences (F: Forward and R: Reverse) and the length of the amplicon in base pairs (bp).
1 Primers described in Dervishi et al. [60].
2 Primers described in García-Crespo et al. 2005 [61].
3 Primers described in Lyahyai et al. 2010 [62].