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. 2012 Sep 22;8:169. doi: 10.1186/1746-6148-8-169

Table 1.

Cell surface, adipogenic, osteogenic, chondrogenic and neurogenic markers analysed by RT-qPCR

Genes
Accession number
Primer sequences
Amplicon size (bp)
  Forward (5’ → 3’) Reverse (5’ → 3’)  
Cell Surface Markers
 
 
 
CD29
AF349461
GTGCCCGAGCCTTCAATAAAG
CCCGATTTTCAACCTTGGTAATG
87
CD34
AB021662
TGGGCATCGAGGACATCTCT
GATCAAGATGGCCAGCAGGAT
107
CD45
NM_001206523
CCTGGACACCACCTCAAAGCT
TCCGTCCTGGGTTTTATCCTG
101
CD73
BC114093
TGGTCCAGGCCTATGCTTTTG
GGGATGCTGCTGTTGAGAAGAA
115
CD90
BC104530
CAGAATACAGCTCCCGAACCAA
CACGTGTAGATCCCCTCATCCTT
96
CD105
NM_001076397
CGGACAGTGACCGTGAAGTTG
TGTTGTGGTTGGCCTCGATTA
115
  Differentiation Markers
 
 
 
PPARG
NM_001100921
GCCCTGGCAAAGCATTTGTA
TGTCTGTCGTCTTTCCCGTCA
94
 
SCD1
AJ001048
CCCAGCTGTCAGAGAAAAGG
GATGAAGCACAACAGCAGGA
115
 
IL6
FJ409227.1
CAGCAAGGAGACACTGGCAG
TGATCAAGCAAATCGCCTGAT
101
 
COL1A1
AF129287
CCTGCGTACAGAACGGCCT
ACAGCACGTTGCCGTTGTC
93
 
BGLAP
DQ418490
CCCAGGAGGGAGGTGTGTG
CTAGACCGGGCCGTAGAAGC
99
 
BGN
NM_001009201.1
AACATGAACTGCATTGAGATGGG
GCGAAGGTAGTTGAGCTTCAGG
93
 
LUM
NM_173934.1
AAGCAATTGAAGAAGCTGCACA
TTAGTGAGCTGCAGGTCCACC
92
 
NES
194665083
CAAATCGCCCAGGTCCTG
GCCTCTAGGAGGGTCCTGTATGT
95
 
NEFM
194669578
GCTCGTCATCTGCGAGAATACC
CACCCTCCAGGAGTTTCCTGTA
91
 
NELF
27806522
CGCTATGCAGGACACAATCAAC
GGGTCTCCTCACCTTCCAAGA
161
 
TUBB3
116004470
GACCTCGAGCCTGGAACCAT
GCCCCACTCTGACCAAAGATG
92
 
MAP2
194664873
TGTCCCAGTGGAGGAAGGTTT
TCTTGTCTAGTGGCTCGGCTG
95
 
PRNP
BC119821
CGCAGAAGCAGGACTTCTGAA
TGGATTTGTGTCTCTGGGAAGA
86
 
Housekeeping genes
 
G6PDH2
AJ507200
TGACCTATGGCAACCGATACAA
CCGCAAAAGACATCCAGGAT
76
 
HPRT3 EF078978 AGGTGTTTATTCCTCATGGAGTAATTATG GGCCTCCCATCTCCTTCATC 79  

GenBank accession numbers of the sequences used for primer design. Primer sequences (F: Forward and R: Reverse) and the length of the amplicon in base pairs (bp).

1 Primers described in Dervishi et al. [60].

2 Primers described in García-Crespo et al. 2005 [61].

3 Primers described in Lyahyai et al. 2010 [62].