Figure 6.

Immunohistochemical staining for intrahepatic CD8-positive, PD-1-positive and PD-L1-positive cells in patients with chronic hepatitis B. Representative figures for patients in T2, T3 and T4 sequence. n = 5, High-power field, original magnification 400×. During T2 period, CD8-positive, PD-1-positive or PD-L1-positive cells infiltrated extensively in liver tissue and they were decreased significantly in T3. In T4, the CD8-positive and PD-1-positive cells were less observed in lobular areas, while PD-L1 positive cells were also frequently observed in liver lobular region.