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Detection of TNF-α and IFN-γ mRNA expression by QT-PCR (n = 5). The levels of TNF-α and IFN-γ mRNA expression significantly increased from T1 to T2, and decreased from T2 to T4. TNF-α expression level in T4 period was still significantly higher than that in T1. TNF-α mRNA was 2.5-fold higher in T4 than that in T1.
