Table 4.
Oligonucleotides used in this study
| Oligo name | Target | Sequencea(5’- 3’)a | Restriction enzyme site |
|---|---|---|---|
| nprE-fw |
nprE |
CGCAAACCATGGGTTTAGGTAAGAAATTGTCTGTTGC |
NcoI |
| nprE-rv |
nprE |
GCGAAATCTAGATTAATGGTGATGGTGATGGTGCAATCCAACAGCATTCCAGGC |
XbaI |
| bla_F |
bla |
AAACCCTCATGAGTATTCAACATTTCCGTGTCG |
PagI |
| bla_R |
bla |
ATACGCTCTAGATTAATGGTGATGGTGATGGTGCCAATGCTTAATCAGTG |
XbaI |
| ycdH-fw |
ycdH |
GCGAAAGGTGACCGATATGTTTAAAAAATGGAGCGG |
BstEII |
| ycdH-rv |
ycdH |
GCGAAATCTAGATTAATGGTGATGGTGATGGTGTGATTTAACCAATAGTGAATCTTTCAGGGC |
XbaI |
| xylP-fw |
xylP |
CGCATATCATGAGCGTTAGTATGCAGC |
PagI |
| xylP-rv | xylP | GCGAAATCTAGATTAATGGTGATGGTGATGGTGCTTTTGATCGTCAGCAA | XbaI |
a Restriction enzyme sites are underlined.