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. 2012 Dec 4;7(12):e50229. doi: 10.1371/journal.pone.0050229

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© 2012 Chen et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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The mRNA expression of Bcl-2 is decreased in HSFBs transfected with Slug shRNA ( = 0.23, s = 0.03) than those in other groups. HSFBs transfected with control shRNA ( = 0.68, s = 0.10) and non-transfected HSFBs ( = 0.70, s = 0.06) expressed the most increased level of Bcl-2 than that in normal skin fibroblasts ( = 0.38, s = 0.05) and HSFBs transfected with Slug shRNA ( = 0.23, s = 0.03) (A and B). Similar with mRNA expression level, the protein expression of Bcl-2 is most decreased in Slug shRNA group ( = 0.24, s = 0.06) and most increased in control shRNA ( = 0.96, s = 0.07) and non-transfected group ( = 0.90, s = 0.15) (C and D). Expression of Bax and PUMA at mRNA and protein level was detected in all groups. The mRNA level of Bax and PUMA was similar among the four groups (A and B). Similarly, western blot (C) and graphic analysis (D) showed that Bax and PUMA were similar in all groups.*: P<0.01 versus Normal skin fibroblast; ^†: P<0.01 versus Control shRNA; ^‡: P<0.01 versus HSFBs.

Inline graphic — The mRNA expression of Bcl-2 is decreased in HSFBs transfected with Slug shRNA ( = 0.23, s = 0.03) than those in other groups. HSFBs transfected with control shRNA ( = 0.68, s = 0.10) and non-transfected HSFBs ( = 0.70, s = 0.06) expressed the most increased level of Bcl-2 than that in normal skin fibroblasts ( = 0.38, s = 0.05) and HSFBs transfected with Slug shRNA ( = 0.23, s = 0.03) (A and B). Similar with mRNA expression level, the protein expression of Bcl-2 is most decreased in Slug shRNA group ( = 0.24, s = 0.06) and most increased in control shRNA ( = 0.96, s = 0.07) and non-transfected group ( = 0.90, s = 0.15) (C and D). Expression of Bax and PUMA at mRNA and protein level was detected in all groups. The mRNA level of Bax and PUMA was similar among the four groups (A and B). Similarly, western blot (C) and graphic analysis (D) showed that Bax and PUMA were similar in all groups.*: P<0.01 versus Normal skin fibroblast; ^†: P<0.01 versus Control shRNA; ^‡: P<0.01 versus HSFBs.