Table 4.
Primary antibodies used in immunohistochemical analysis
| MOLECULE | TYPE OF Ab | COMPANY | WORKING DILUTION | POSITIVE CONTROL |
|---|---|---|---|---|
| β-catenin |
Rabbit PAb |
Santa Cruz Biotecnology |
1:250 |
Normal canine skin [80] |
| Survivin |
Rabbit PAb |
NOVUS Biologicals |
0.7 μg/ml |
Canine cutaneous SCC [79] |
| p53 |
Rabbit PAb |
NovoCastra |
1:400 |
Canine nail bed SCC (Figure 5) |
| Procaspase 3 |
Rat MAb |
NovoCastra |
1:85 |
Normal canine tonsil [81] |
| Active Caspase 3 | Mouse MAb | Oncogene | 1:200 | Canine breast carcinoma [81] |
Clonality, company, working dilutions and positive controls used for each antibody. PAb: polyclonal antibody; MAb: monoclonal antibody. SCC: squamous cell carcinoma.