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. Author manuscript; available in PMC: 2013 Sep 1.
Published in final edited form as: Arterioscler Thromb Vasc Biol. 2012 Jul 5;32(9):2214–2222. doi: 10.1161/ATVBAHA.112.252015

Figure 5.

Figure 5

Bmper is required for the regulation of ICAM1 and VCAM1 expression by fluid shear stress. A, HUVECs were subjected to oscillatory shear stress (OS, ±5 dyn cm-2) or laminar shear stress (LS, 20 dyn cm-2) or remained static in the incubator for 8 hours. Cell lysates were used for Western blotting to detect the expression of Bmper and Bmp4 and the induction of inflammatory adhesion molecules ICAM1 and VCAM1. B, The band intensity of Bmp4 and Bmper (A) was quantified with ImageJ and is presented as the ratio of Bmp4/Bmper protein level compared to the LS condition. *, P<0.05 compared to the cells exposed to laminar shear stress. n=4. C, HUVECs were transfected with Bmper or control siRNAs. Four days later, cells were exposed to oscillatory shear stress (±5 dyn cm-2), laminar shear stress (20 dyn cm-2) or static conditions for 8 hours. D~E, The band intensity of ICAM1 (D) and VCAM1 (E) was quantified with ImageJ and is presented as the relative fold change of protein level compared to the LS condition. *, P<0.05 compared to the cells transfected with control siRNAs, n=3; #, P<0.05 compared to the cells exposed to laminar shear stress, n=3. F, HUVECs were transfected with Bmper or control siRNAs. Four days later, cells were exposed to oscillatory shear stress (±5 dyn cm-2), laminar shear stress (20 dyn cm-2) or static conditions for 8 hours. Cell lysates were subjected for Western blotting with phospho-eNOS (Ser1177) and eNOS antibodies. G, The band intensity of phospho-eNOS (Ser1177)(E) was quantified with ImageJ and is presented as the relative fold change of protein level compared to the total eNOS protein level. *, P<0.02 compared to the cells at the static condition; #, P<0.03 compared to the cells transfected with control siRNA. n=3.