Skip to main content
. Author manuscript; available in PMC: 2013 Mar 16.
Published in final edited form as: Circ Res. 2012 Feb 21;110(6):808–817. doi: 10.1161/CIRCRESAHA.111.259325

Figure 2. TUPS prevents Hcy-induced reduction in 14,15-EET and endothelial activation.

Figure 2

HUVECs (5×105) were incubated with PBS, Hcy (200µmol/L), or plus TUPS (1µmol/L) or 14,15-EET (100 nmol/L) for 24 hr. Intracellular levels of 14,15-EET and 14,15-EET/DHET were detected by ELISA (A, B). Quantitative RT-PCR of mRNA expression (C, D) or western blot analysis (E) of protein expression of VCAM-1 or ICAM-1. (F) Quantification of protein levels was by densitometry. Data are means±SD from 3 dependent experiment. *P<0.05, **P<0.01 vs. PBS controls, # P<0.05 vs. Hcy.