Spinal cord injury increased full-length EphA7 protein levels. SDS-PAGE showed a unique band at 120 kDa region for full length EphA7 compared with similar fractions that were incubated with the antibody pre-absorbed with the antigen used to generate it (A). Time course studies after SCI demonstrated that EphA7 immunoreactive protein increased relative to sham control, with no changes observed with β-actin expression (B). Densitometry analysis demonstrated a significant upregulation at 2 (*p<0.05), 4 (*p<0.05), 14 (*p<0.05) and 28 DPI (**p<0.01), n= 3 (C). ANOVA followed by Dunnett test was used to determine the significant differences among samples studies (F(5,12) = 6.061, p<0.0019).